eLife | |
Pre-post synaptic alignment through neuroligin-1 tunes synaptic transmission efficiency | |
Thomas M Bartol1  Benjamin Compans1  Olivier Thoumine1  Terrence J Sejnowski1  Matthieu Sainlos2  Eric Hosy2  Daniel Choquet3  Kalina T Haas3  Dolors Grillo-Bosch3  Mathieu Letellier3  | |
[1] Interdisciplinary Institute for Neuroscience, CNRS, UMR 5297, F-33000, Bordeaux, France;Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, United States;Interdisciplinary Institute for Neuroscience, University of Bordeaux, UMR 5297, F-33000, Bordeaux, France; | |
关键词: AMPA receptors; super-resolution microscopy; synaptic transmission; | |
DOI : 10.7554/eLife.31755 | |
来源: DOAJ |
【 摘 要 】
The nanoscale organization of neurotransmitter receptors regarding pre-synaptic release sites is a fundamental determinant of the synaptic transmission amplitude and reliability. How modifications in the pre- and post-synaptic machinery alignments affects synaptic currents, has only been addressed with computer modelling. Using single molecule super-resolution microscopy, we found a strong spatial correlation between AMPA receptor (AMPAR) nanodomains and the post-synaptic adhesion protein neuroligin-1 (NLG1). Expression of a truncated form of NLG1 disrupted this correlation without affecting the intrinsic AMPAR organization, shifting the pre-synaptic release machinery away from AMPAR nanodomains. Electrophysiology in dissociated and organotypic hippocampal rodent cultures shows these treatments significantly decrease AMPAR-mediated miniature and EPSC amplitudes. Computer modelling predicts that ~100 nm lateral shift between AMPAR nanoclusters and glutamate release sites induces a significant reduction in AMPAR-mediated currents. Thus, our results suggest the synapses necessity to release glutamate precisely in front of AMPAR nanodomains, to maintain a high synaptic responses efficiency.
【 授权许可】
Unknown