Genomic Changes within a Subset of IncI2 Plasmids Associated with Dissemination of mcr-1 Genes and Other Important Antimicrobial Resistance Determinants

Antibiotics

Gabhan Chalmers¹  Heather K. Allen²  Nicole Ricker²  Elli Whalen²  Richard J. Meinersmann³ 

[1] Department of Pathobiology, University of Guelph, Guelph, ON N1G 2W1, Canada;Food Safety and Enteric Pathogens Research Unit, ARS-USDA National Animal Disease Center, Ames, IA 50010, USA;National Poultry Research Center, USDA Agricultural Research Service, PMSP-RU, Athens, GA 30605, USA;

关键词: mcr-1;    IncI2;    antibiotic resistance;    insertion sequences;    beta-lactamase;    plasmids;   

DOI  :  10.3390/antibiotics11020181

来源: DOAJ

【 摘 要 】

IncI2 plasmids appear to have only recently become associated with resistance genes; however, their tendency to carry resistance to the antibiotics of last resort and their widespread distribution increase their relative importance. In this study, we describe lineages within this plasmid family that have an increased likelihood of acquisition of antimicrobial resistance genes. Globally distributed mcr-1-carrying IncI2 plasmids were found to cluster with other IncI2 plasmids carrying extended-spectrum beta-lactamase genes, and separately from the non-resistant IncI2 plasmids. In addition, insertion sequence (IS) elements with no direct association with the acquired resistance genes also clustered with the resistance plasmids in the phylogenetic tree. In recognition of the biased sequencing of resistant plasmids globally, the analysis was also performed on resistant and non-resistant IncI2 plasmids sequenced in the USA through government surveillance efforts that do not rely on antibiotic selection. This analysis confirmed a distinct clustering associated with both resistance and mobile elements and identified possible genomic changes in core genes that correlate with increased acquisition of foreign DNA. This work highlights a potential genetic mechanism for increased uptake of foreign DNA within this prevalent family of plasmids.

【 授权许可】

Unknown