Systematic characterization of the branch point binding protein, splicing factor 1, gene family in plant development and stress responses

BMC Plant Biology

Kai-Lu Zhang¹  Yan-Ming Fang¹  Fu-Yuan Zhu¹  Zhen Feng²  Caie Wu²  Jing-Fang Yang³  Ge-Fei Hao³  Mo-Xian Chen⁴  Feng Yang⁵  Jianhua Zhang⁵  Tian Yuan⁵  Di Zhang⁵ 

[1] Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University;College of Light Industry and Food Engineering, Nanjing Forestry University;Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University;Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences;Shenzhen Research Institute, The Chinese University of Hong Kong;

关键词: Alternative splicing;    Expression profile;    Phylogenetics;    Plants;    Promoter;    Splicing factor;   

DOI  :  10.1186/s12870-020-02570-6

来源: DOAJ

【 摘 要 】

Abstract Background Among eukaryotic organisms, alternative splicing is an important process that can generate multiple transcripts from one same precursor messenger RNA, which greatly increase transcriptome and proteome diversity. This process is carried out by a super-protein complex defined as the spliceosome. Specifically, splicing factor 1/branchpoint binding protein (SF1/BBP) is a single protein that can bind to the intronic branchpoint sequence (BPS), connecting the 5′ and 3′ splice site binding complexes during early spliceosome assembly. The molecular function of this protein has been extensively investigated in yeast, metazoa and mammals. However, its counterpart in plants has been seldomly reported. Results To this end, we conducted a systematic characterization of the SF1 gene family across plant lineages. In this work, a total of 92 sequences from 59 plant species were identified. Phylogenetic relationships of these sequences were constructed, and subsequent bioinformatic analysis suggested that this family likely originated from an ancient gene transposition duplication event. Most plant species were shown to maintain a single copy of this gene. Furthermore, an additional RNA binding motif (RRM) existed in most members of this gene family in comparison to their animal and yeast counterparts, indicating that their potential role was preserved in the plant lineage. Conclusion Our analysis presents general features of the gene and protein structure of this splicing factor family and will provide fundamental information for further functional studies in plants.

【 授权许可】

Unknown