Muscle-specific myosin heavy chain shifts in response to a long-term high fat / high sugar diet and resveratrol treatment in nonhuman primates

Frontiers in Physiology

Lisa eNguyen¹  Robert J. Talmadge¹  Jeannine R. Larocque²  Jon-Philippe K. Hyatt²  Allison E. Hall²  Ashley M. Huber²  Jessica C. Kocan²  Rafael ede Cabo³  Julie A. Mattison³ 

[1] California State Polytechnic University;Georgetown University;National Institutes of Health;

关键词: skeletal muscle;    MHC;    GLUT4;    rhesus macaque;    PGC-1α;   

DOI  :  10.3389/fphys.2016.00077

来源: DOAJ

【 摘 要 】

Shifts in myosin heavy chain (MHC) expression within skeletal muscle can be induced by a host of stimuli including, but not limited to, physical activity, alterations in neural activity, aging, and diet or obesity. Here, we hypothesized that both age and a long-term (2 year) high fat / high sugar diet (HFS) would induce a slow to fast MHC shift within the plantaris, soleus, and extensor digitorum longus (EDL) muscles from rhesus monkeys. Furthermore, we tested whether supplementation with resveratrol, a naturally occurring compound that has been attributed with augmenting aerobic potential through mitochondrial proliferation, would counteract any diet-induced MHC changes by promoting a fast to slow isoform switch.In general, we found that MHC isoforms were not altered by aging during mid-life. The HFS diet had the largest impact within the soleus muscle where the greatest slow to fast isoform shifts were observed in both mRNA and protein indicators. As expected, long-term resveratrol treatment counteracted, or blunted, these diet-induced shifts within the soleus muscle. The plantaris muscle also demonstrated a fast-to-slow phenotypic response to resveratrol treatment.In conclusion, diet or resveratrol treatment impacts skeletal muscle phenotype in a muscle-specific manner and resveratrol supplementation may be one approach for promoting the fatigue-resistant MHC (type I) isoform especially if its expression is blunted as a result of a long-term high fat/sugar diet.

【 授权许可】

Unknown