Enterovirus D68 in Viet Nam (2009-2015) [version 2; referees: 3 approved]

Wellcome Open Research

VIZIONS Consortium¹  Ngo Ngoc Quang Minh²  Ha Manh Tuan³  Tran Thi Thuy Minh⁴  Nguyen Van Hung⁴  Tran Quoc Loi⁵  Nguyen Tran Anh Vu⁵  Nguyen Van Vinh Chau⁶  Nguyen Van Xang⁷  Nguyen Dong⁷  Do Lien Anh Ha⁸  Angela McBride⁹  Tan Le Van⁹  Pham Thi Thanh Tam⁹  Vu Thi Ty Hang⁹  Nguyen Thi Han Ny⁹  Tran My Phuc⁹  Nguyen To Anh⁹  Dang Thao Huong⁹  Ho Dang Trung Nghia⁹  Stephen Baker⁹  H. Rogier van Doorn⁹  Tran Tan Thanh⁹  Do Quang Ha⁹  Guy Thwaites⁹  Lam Anh Nguyet⁹  Catherine Anscombe⁹ 

[1] ;Children's Hospital 1, Ho Chi Minh City, Vietnam;Children's Hospital 2, Ho Chi Minh City, Vietnam;Dak Lak General Hospital, Ban Me Thuot City, Vietnam;Dong Thap General Hospital, Ban Me Thuot City, Vietnam;Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam;Khanh Hoa General Hospital, Nha Trang City, Vietnam;Murdoch Children's Research Institute, Melbourne, Australia;Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam;

关键词: Viral Infections (without HIV);    Virology;   

DOI  :  10.12688/wellcomeopenres.11558.2

来源: DOAJ

【 摘 要 】

Background: Since 1962, enterovirus D68 (EV-D68) has been implicated in multiple outbreaks and sporadic cases of respiratory infection worldwide, especially in the USA and Europe with an increasing frequency between 2010 and 2014. We describe the detection, associated clinical features and molecular characterization of EV-D68 in central and southern Viet Nam between 2009 and 2015. Methods: Enterovirus/rhinovirus PCR positive respiratory or CSF samples taken from children and adults with respiratory/central nervous system infections in Viet Nam were tested by an EV-D68 specific PCR. The included samples were derived from 3 different observational studies conducted at referral hospitals across central and southern Viet Nam 2009  2015. Whole-genome sequencing was carried out using a MiSeq based approach. Phylogenetic reconstruction and estimation of evolutionary rate and recombination were carried out in BEAST and Recombination Detection Program, respectively. Results: EV-D68 was detected in 21/625 (3.4%) enterovirus/rhinovirus PCR positive respiratory samples but in none of the 15 CSF. All the EV-D68 patients were young children (age range: 11.8 – 24.5 months) and had moderate respiratory infections. Phylogenetic analysis suggested that the Vietnamese sequences clustered with those from Asian countries, of which 9 fell in the B1 clade, and the remaining sequence was identified within the A2 clade. One intra sub-clade recombination event was detected, representing the second reported recombination within EV-D68. The evolutionary rate of EV-D68 was estimated to be 5.12E -3 substitutions/site/year. Phylogenetic analysis indicated that the virus was imported into Viet Nam in 2008. Conclusions: We have demonstrated for the first time EV-D68 has been circulating at low levels in Viet Nam since 2008, associated with moderate acute respiratory infection in children. EV-D68 in Viet Nam is most closely related to Asian viruses, and clusters separately from recent US and European viruses that were suggested to be associated with acute flaccid paralysis.

【 授权许可】

Unknown