Emergence of blaNDM– 1-Carrying Aeromonas caviae K433 Isolated From Patient With Community-Acquired Pneumonia

Frontiers in Microbiology

Kai Mu²  Dongguo Wang³  Yujie Zhao⁴  Yifan Jia⁵  Lianhua Yu⁵  Xinhua Luo⁵  Jin Zhang⁵  Dakang Hu⁵  Piaopiao Dai⁵  Ying Qu⁵  Jian Weng⁶ 

[1] Beijing Institute of Radiation Medicine, Beijing, China;Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Diseases, Beijing, China;Department of Central Laboratory, Taizhou Municipal Hospital Affiliated With Taizhou University, Taizhou, China;Department of Clinical Laboratory Medicine, Ningbo Medical Center Li Huili Hospital, Ningbo, China;Department of Clinical Laboratory Medicine, Taizhou Municipal Hospital Affiliated With Taizhou University, Taizhou, China;Taizhou Center for Disease Control and Prevention, Taizhou, China;

关键词: Aeromonas spp.;    IMEs;    mobile genetic elements;    blaNDM;    multidrug resistance;   

DOI  :  10.3389/fmicb.2022.825389

来源: DOAJ

【 摘 要 】

To demonstrate the detailed genetic characteristics of a blaNDM–1-carrying multidrug-resistant Aeromonas caviae strain, the complete genome of the A. caviae strain K433 was sequenced by Illumina HiSeq and Oxford nanopore platforms, and mobile genetic elements associated with antibiotic resistance genes were analyzed by a series of bioinformatics methods. A. caviae K433 which was determined to produce class B carbapenemase, was resistant to most antibiotics tested except amikacin. The genome of K433 consisted of a chromosome cK433 (6,482-kb length) and two plasmids: pK433-qnrS (7.212-kb length) and pK433-NDM (200.855-kb length), the last being the first investigated blaNDM-carrying plasmid from Aeromonas spp. By comparison of the backbone and MDR regions from the plasmids studied, they involved a highly homologous sequence structure. This study provides in-depth genetic insights into the plasmids integrated with blaNDM-carrying genetic elements from Aeromonas spp.

【 授权许可】

Unknown