Disclosing the Nutritional Quality Diversity of Portuguese Common Beans—The Missing Link for Their Effective Use in Protein Quality Breeding Programs

Agronomy

Mariade Fátima Cabral¹  Judite Costa¹  MariaE. Figueira¹  Diego Rubiales²  Bruna Carbas³  Carla Brites³  MariaManuela Veloso³  AndreiaBento da Silva⁴  SusanaT. Leitão⁴  Sofia Natalello⁴  Elsa Mecha⁴  MariaC. Vaz Patto⁴  MariaR. Bronze⁴ 

[1] Faculdade de Farmácia, Universidade de Lisboa, Av. das Forças Armadas, 1649-019 Lisboa, Portugal;IAS, Institute for Sustainable Agriculture, CSIC, Avda Menéndez Pidal s/n, 14004 Córdoba, Spain;INIAV, Instituto Nacional de Investigação Agrária e Veterinária, Av. da República, 2784-505 Oeiras, Portugal;ITQB NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal;

关键词: common bean;    variability;    food sustainability;    nutritional value;    protein quality;    amino acid;   

DOI  :  10.3390/agronomy11020221

来源: DOAJ

【 摘 要 】

The common bean (Phaseolus vulgaris L.) represents a sustainable and affordable source of protein, namely, to populations with vegetarian dietary habits. Despite the national germplasm genetic diversity, little is known about the Portuguese accessions’ nutritional and protein quality, leading to their underuse in breeding programs. To fill this gap, a representative collection (106 accessions) was cropped under two contrasting environments (traditional versus heat stress) and evaluated in terms of nutritional quality by near-infrared spectroscopy. Protein quality was assessed, under the stressful environment, considering the individual amino acid contents and the activity of trypsin inhibitors through mass spectrometry (LC-MS/MS) and spectrophotometry, respectively. On top of strong genotypic control, the nutritional composition (protein, fat, fiber, moisture and ash) was also highly influenced by the environment and by genotype × environment interaction, with a clear nutritional quality ranking change for the accessions in heat stress conditions. Classified into three clusters, the accessions from the cluster with the highest individual amino acid and protein contents also showed higher trypsin inhibitor activity (TIA). Since different levels of TIA had no translation into contrasting protein digestibility, breeders focusing on common beans’ protein quality improvement, especially under challenging warming climate conditions, may take advantage of this group of accessions.

【 授权许可】

Unknown