Optimization of Specifications for Scaled-Up Fixed Rabies Virus Cultivation (“Moscow 3253” Strain) in Vero Cell Culture

Проблемы особо опасных инфекций

Zh. V. Matveeva¹  S. V. Generalov¹  O. A. Lobovikova¹  E. G. Abramova¹  I. M. Zhulidov¹  L. V. Savitskaya¹ 

[1] Russian Research Anti-Plague Institute “Microbe”;

关键词: бешенство;    культура клеток;    роллерное выращивание;    антирабический иммуноглобулин;    rabies;    cell culture;    roll-bottle cultivation;    anti-rabies immunoglobulin;   

DOI  :  10.21055/0370-1069-2014-2-101-103

来源: DOAJ

【 摘 要 】

/cell, using maintenance media 199 with admixture of 0.1 % human serum albumin or 2 % bovine serum. Optimum media volume in the roll-bottle for fixed rabies virus strain, “Moscow 3253”, cultivation is 200-400 ml. It depends upon the proliferating surface area. Specifications stated above provide for the obtainment of culture liquid with rabies virus titer - 1:256 - 1:512, if assayed in ELISA. This cultural virus is recommended as a basis for immunization material obtainment with a view to produce anti-rabies immunoglobulin from equine blood serum.

【 授权许可】

Unknown