Putative Periodontal Pathogens, Filifactor alocis and Peptoanaerobacter stomatis, Induce Differential Cytokine and Chemokine Production by Human Neutrophils

Pathogens

RichardJ. Lamont¹  Emeri Jimenez-Flores²  Aruna Vashishta²  Shifu Tian²  ChristopherK. Klaes²  Irina Miralda³  SilviaM. Uriarte⁴ 

[1] Immunology, School of Medicine, University of Louisville, 505 S. Hancock St., Louisville, KY 40202, USA;Department of Medicine, School of Medicine, University of Louisville, 570 S. Preston St., Louisville, KY 40202, USA;;Department of Microbiology &Department of Oral Immunology and Infectious Diseases, School of Dentistry, University of Louisville, 570 S. Preston St., Louisville, KY 40202, USA;

关键词: Emerging oral pathogens;    human neutrophils;    cytokines and chemokines;    chemotaxis;   

DOI  :  10.3390/pathogens8020059

来源: DOAJ

【 摘 要 】

Periodontitis is a highly prevalent infectious disease that affects ~ 50% of the adults in the USA alone. Two Gram-positive anaerobic oral bacteria, Filifactor alocis and Peptoanaerobacter stomatis, have emerged as important periodontal pathogens. Neutrophils are a major component of the innate host response in the gingival tissue, and the contribution of neutrophil-derived cytokines and chemokines plays a central role in disease progression. The pattern of cytokines and chemokines released by human neutrophils upon stimulation with newly appreciated periodontal bacteria compared to the keystone oral pathogen Porphyromonas gingivalis was investigated. Our results showed that both F. alocis and P. stomatis triggered TLR2/6 activation. F. alocis induced significant changes in gene expression of cytokines and chemokines in human neutrophils compared to unstimulated cells. However, except for IL-1ra, neutrophils released lower levels of cytokines and chemokines in response to F. alocis compared to P. stomatis. Furthermore, bacteria-free conditioned supernatant collected from neutrophils challenged with P. stomatis, but not from P. gingivalis or F. alocis, was chemotactic towards both neutrophils and monocytes. Elucidating stimuli-specific modulation of human neutrophil effector functions in the context of dysbiotic microbial community constituents provides valuable information for understanding the pathogenesis of periodontal diseases.

【 授权许可】

Unknown