The Research of Toxicity and Sensitization Potential of PEGylated Silver and Gold Nanomaterials

Toxics

Jun-Young Yang¹  Jae-Ho Oh¹  Ki-Kyung Jung¹  Seo-Yoon Choi¹  Sung-Hyun Kim¹  Jin-Hee Lee¹  Dong-Han Lee¹  Ja-young Jeong¹ 

[1] Division of Toxicological Research, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju 28159, Korea;

关键词: skin sensitization;    immunogenicity;    alternative test;    nanomaterials;    polyethylene glycol;    PEGylation;   

DOI  :  10.3390/toxics9120355

来源: DOAJ

【 摘 要 】

Polyethylene glycol (PEG) is a polymer used for surface modification of important substances in the modern pharmaceutical industry and biopharmaceutical fields. Despite the many benefits of PEGylation, there is also the possibility that the application and exposure of the substance may cause adverse effects in the body, such as an immune response. Therefore, we aimed to evaluate the sensitization responses that could be induced through the intercomparison of nanomaterials of the PEG-coated group with the original group. We selected gold/silver nanomaterials (NMs) for original group and PEGylated silver/gold NMs in this study. First, we measured the physicochemical properties of the four NMs, such as size and zeta potential under various conditions. Additionally, we performed the test of the NM’s sensitization potential using the KeratinoSens™ assay for in vitro test method and the LLNA: 5-bromo-2-deoxyuridine (BrdU)-FCM for in vivo test method. The results showed that PEGylated-NMs did not lead to skin sensitization according to OECD TG 442 (alternative test for skin sensitization). In addition, gold nanomaterial showed that cytotoxicity of PEGylated-AuNMs was lower than AuNMs. These results suggest the possibility that PEG coating does not induce an immune response in the skin tissue and can lower the cytotoxicity of nanomaterials.

【 授权许可】

Unknown