Metabolomic Profiles in Adipocytes Differentiated from Adipose-Derived Stem Cells Following Exercise Training or High-Fat Diet

International Journal of Molecular Sciences

Junetsu Ogasawara¹  Seita Osawa²  Yuki Maeda²  Hisashi Takakura²  Hisashi Kato²  Tetsuya Izawa² 

[1] Division of Health Science, Asahikawa Medical University, 2–1-1–1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078–8510, Japan;Graduate School of Health and Sports Science, Doshisha University, 1–3 Tatara-Miyakodani, Kyotanabe City, Kyoto 610–0394, Japan;

关键词: adipose-derived stem cell;    exercise training;    amino acids;    perigonadal adipose tissue;    subcutaneous adipose tissue;    metabolome;   

DOI  :  10.3390/ijms22020966

来源: DOAJ

【 摘 要 】

Controlling the differentiation potential of adipose-derived stem cells (ADSCs) is attracting attention as a new strategy for the prevention and treatment of obesity. Here, we aimed to observe the effect of exercise training (TR) and high-fat diet (HFD) on the metabolic profiles of ADSCs-derived adipocytes. The rats were divided into four groups: normal diet (ND)-fed control (ND-SED), ND-fed TR (ND-TR), HFD-fed control (HFD-SED), and HFD-fed TR (HFD-TR). After 9 weeks of intervention, ADSCs of epididymal and inguinal adipose tissues were differentiated into adipocytes. In the metabolome analysis of adipocytes after isoproterenol stimulation, 116 metabolites were detected. The principal component analysis demonstrated that ADSCs-derived adipocytes segregated into four clusters in each fat pad. Amino acid accumulation was greater in epididymal ADSCs-derived adipocytes of ND-TR and HFD-TR, but lower in inguinal ADSCs-derived adipocytes of ND-TR, than in the respective controls. HFD accumulated several metabolites including amino acids in inguinal ADSCs-derived adipocytes and more other metabolites in epididymal ones. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that TR mainly affected the pathways related to amino acid metabolism, except in inguinal ADSCs-derived adipocytes of HFD-TR rats. These findings provide a new way to understand the mechanisms underlying possible changes in the differentiation of ADSCs due to TR or HFD.

【 授权许可】

Unknown