Molecular Strategies for Intensity-Dependent Olfactory Processing in Caenorhabditis elegans

Frontiers in Molecular Neuroscience

Shumin Duan²  Jiajie Shao³  Zhibing Tan³  Yadan Xue³  Siyan Liu⁴  Lijun Kang⁴  Hankui Cheng⁴  Yu Liu⁴ 

[1] Department of Neurobiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China;Department of Neurology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China;Department of Neurosurgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China;NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Research and Brain-Machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, China;

关键词: olfactory processing;    cGMP;    intensity dependence;    sensory neuron;    calcium imaging assay;   

DOI  :  10.3389/fnmol.2021.748214

来源: DOAJ

【 摘 要 】

Various odorants trigger complex animal behaviors across species in both quality- and quantity-dependent manners. However, how the intensity of olfactory input is encoded remains largely unknown. Here we report that isoamyl alcohol (IAA) induces bi-directional currents through a Gα- guanylate cyclase (GC)- cGMP signaling pathway in Caenorhabditis elegans olfactory neuron amphid wing “C” cell (AWC), while two opposite cGMP signaling pathways are responsible for odor-sensing in olfactory neuron amphid wing “B” cell (AWB): (1) a depolarizing Gα (GPA-3)- phosphodiesterase (PDE) – cGMP pathway which can be activated by low concentrations of isoamyl alcohol (IAA), and (2) a hyperpolarizing Gα (ODR-3)- GC- cGMP pathway sensing high concentrations of IAA. Besides, IAA induces Gα (ODR-3)-TRPV(OSM-9)-dependent currents in amphid wing “A” cell (AWA) and amphid neuron “H” cell with single ciliated sensory ending (ASH) neurons with different thresholds. Our results demonstrate that an elaborate combination of multiple signaling machineries encode the intensity of olfactory input, shedding light on understanding the molecular strategies on sensory transduction.

【 授权许可】

Unknown