Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection

Crystals

Asim Jilani¹  Javed Iqbal¹  Sami Ullah²  Hafiz Zeeshan Mahmood³  Swelm Wageh⁴  Yasir Javed⁵  Yasir Jamil⁵  Sajid Farooq⁶ 

[1] Center of Nanotechnology, King Abdulaziz University, Jeddah 21589, Saudi Arabia;Department of Chemistry, Faculty of Science, Research Center for Advanced Materials Science (RCAMS), King Khalid University, Abha 61413, Saudi Arabia;Department of Electrical Emgineering and Systems, Universidade Federal de Pernambuco, Recife 50670-901, Brazil;Department of Physics, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia;Department of Physics, University of Agriculture, Faisalabad 38040, Pakistan;Escola Politécnica de Pernambuco—POLI, Universidade de Pernambuco, Recife 50100-010, Brazil;

关键词: localized surface plasmon resonance;    gold nanoparticle;    plasmonic sensor;    dengue detection;   

DOI  :  10.3390/cryst11111340

来源: DOAJ

【 摘 要 】

In this study, a novel label-free immunosensor platform is developed to exploit the localized surface plasmon resonance (LSPR) phenomenon. The LSPR solution-based platform is designed by a gold nanospheres probe, functionalized with monoclonal anti-dengue antibody (IgG). Numerical calculations are performed to assess the LSPR extinction spectrum and spatial near electric field distribution around the nanoparticle surface. Important parameters that govern sensor performance, molecular and refractive index sensitivity are evaluated. On the evaluation of the platform as a molecular sensor, the detection of dengue NS1 antigens is presented. The results are consistent with the numerical simulations, which depicts the system’s ability to identify dengue NS1 antigen concentrations as low as 0.07 ± 0.01 µg/mL, along with fosters its potential application in plasmonic sensing.

【 授权许可】

Unknown