Lactobacillus Plantarum 108 Inhibits Streptococcus mutans and Candida albicans Mixed-Species Biofilm Formation

Antibiotics

Tomoko Ohshima¹  Louis Yi Ann Chai²  Neha Srivastava³  Kassapa Ellepola³  Nityasri Venkiteswaran³  Chaminda Jayampath Seneviratne⁴ 

[1] Department of Oral Microbiology, School of Dental Medicine, Tsurumi University, Yokohama 230-8501, Japan;Division of Infectious Diseases, University Medicine Cluster, National University Health System and Faculty of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Singapore;Oral Sciences, Faculty of Dentistry, National University of Singapore, Singapore 11908, Singapore;Singapore Oral Microbiomics Initiative, National Dental Research Institute Singapore (NDRIS), National Dental Centre Singapore, SingHealth Duke NUS Medical School, 5 Second Hospital Avenue, Singapore 168938, Singapore;

关键词: Streptococcus mutans;    Lactobacillus plantarum;    glucosyltransferase;    dental caries;    probiotics;   

DOI  :  10.3390/antibiotics9080478

来源: DOAJ

【 摘 要 】

Streptococcus mutans is the principal biofilm forming oral pathogen associated with dental caries. Studies have shown that Candida albicans, a commensal oral fungus is capable of forming pathogenic mixed-species biofilms with S. mutans. The treatment of bacterial and fungal infections using conventional antimicrobial agents has become challenging due to the antimicrobial resistance of the biofilm mode of growth. The present study aimed to evaluate the efficacy of secretory components of Lactobacillus plantarum 108, a potentially promising probiotic strain, against S. mutans and C. albicans single and mixed-species biofilms. L. plantarum 108 supernatant inhibited S. mutans and C. albicans single-species biofilms as shown by XTT reduction assay, crystal violet assay, and colony forming units counting. The probiotic supernatant significantly inhibited the S. mutans and C. albicans mixed-species biofilm formation. The pre-formed mixed-species biofilms were also successfully reduced. Confocal microscopy showed poorly developed biofilm architecture in the probiotic supernatant treated biofilms. Moreover, the expression of S. mutans genes associated with glucosyltransferase activity and C. albicans hyphal specific genes (HWP1, ALS1 and ALS3) were down-regulated in the presence of the probiotic supernatant. Altogether, the data demonstrated the capacity of L. plantarum 108 supernatant to inhibit the S. mutans and C. albicans mixed-species biofilms. Herein, we provide a new insight on the potential of probiotic-based strategies to prevent bacterial-fungal mixed-species biofilms associated with dental caries.

【 授权许可】

Unknown