Integrated Bacteria-Fungi Diversity Analysis Reveals the Gut Microbial Changes in Buffalo With Mastitis

Frontiers in Veterinary Science

Kun Duan¹  Lingtong Quan²  Muhammad Fakhar-e-Alam Kulyar³  Wenqian Zhang³  Miao An³  Xin Wan⁴  Hui Zhou⁴  Yu Wu⁴  Jianlong Li⁵  Zhanhai Mai⁵  Xiushuang Chen⁶  Kun Li⁶  Yi Wu⁶  Wenxia Bai⁷ 

[1] China Tobacco Henan Industrial Co. Ltd., Zhengzhou, China;College of Life Sciences, Nanjing Agricultural University, Nanjing, China;College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China;College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China;College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, China;MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China;Nanjing Superbiotech Co. Ltd., Nanjing, China;

关键词: gut microbiota;    bacterial;    fungal;    buffalo;    mastitis;   

DOI  :  10.3389/fvets.2022.918541

来源: DOAJ

【 摘 要 】

The gut microbial community is closely related to mastitis, but studies regarding the influences of mastitis on gut microbiota in buffalo remain scarce. Herein, we characterized the differences in gut bacterial and fungal communities between mastitis-affected and healthy buffalos. Interestingly, although mastitis had no effect on gut bacterial and fungal diversities in the buffalos, some bacterial and fungal taxa were significantly altered. Bacterial and fungal taxonomic analysis showed that the preponderant bacterial phyla (Firmicutes and Bacteroidetes) and fungal phyla (Ascomycota and Basidiomycota) in buffalo were the same regardless of health status. At the level of genus, the changes in some gut bacterial and fungal abundances between both groups were gradually observed. Compared with healthy buffalos, the proportions of 3 bacterial genera (uncultured_bacterium_f_Muribaculaceae, Eubacterium_nodatum_group, and Lachnoclostridium_10) and 1 fungal genus (Pichia) in the mastitis-affected buffalo were significantly increased, whereas 4 bacterial genera (Ruminococcus_2, Candidatus_Stoquefichus, Turicibacter, and Cellulosilyticum) and 4 fungal genera (Cladosporium, Thermothelomyces, Ganoderma and Aspergillus) were significantly decreased. Taken together, this research revealed that there was significant difference in the compositions of the gut microbial community between the healthy and mastitis-affected buffalos. To our knowledge, this is the first insight into the characteristics of the gut microbiota in buffalos with mastitis, which is beneficial to understand the gut microbial information of buffalo in different health states and elucidate the pathogenesis of mastitis from the gut microbial perspective.

【 授权许可】

Unknown