Impairment of maturation of BMP-6 (35 kDa) correlates with delayed fracture healing in experimental diabetes

Journal of Orthopaedic Surgery and Research

Zheng Guo¹  Weijie Wang²  Rami Abboud²  Qidong Guo³ 

[1] Department of Anaesthesia, Second Hospital of Shanxi Medical University;Department of Orthopaedic and Trauma Surgery, Medical School, University of Dundee;Department of Orthopaedics, Second Hospital of Shanxi Medical University;

关键词: BMP-6;    Delayed union;    Diabetes;    Fracture;    Healing;    Nonunion;   

DOI  :  10.1186/s13018-020-01705-7

来源: DOAJ

【 摘 要 】

Abstract Background Although it is known that diabetes interferes with fracture healing, the mechanisms remain poorly understood. The aim of this study was to investigate the correlation of BMP-6 and BMP-9 with the impairment in fracture healing in diabetes, by analyses of the difference in size and calcification of the callus, mechanical endurance, and expressing BMP-6 and BMP-9 in the callus, using a clinical related diabetic rodent model. Methods We evaluated femur fracture healing by quantification of size and calcification of the callus by X-ray, histological and histochemical images, loading capacity of the fractured bone, and amount of BMP-6 in the callus and the bones using Western blot assay. Results Significant upregulation of BMP-6 in the callus and the fractured bones of both non-diabetic and the diabetic animals was observed, at the end of the second and the fourth weeks after fracture. However, significantly lower levels of BMP-6 at 35 kDa with smaller sizes of calcified callus and poor loading capacity of the healing bones were detected in the diabetic animals, compared to the non-diabetic controls. The impairment of the maturation procedure of BMP-6 (35 kDa) from precursors may be underlying the downregulation of the BMP-6 in diabetic animals. Conclusions It could be concluded that the delayed fracture healing in the diabetic animals is correlated with deficiency of BMP-6 (35 kDa), which may be caused by impairment of maturation procedure of BMP-6 from precursors to functioning format. This is a primary study but an important step to explore the molecular pathogenesis of impairment of fracture healing in diabetes and to molecular therapeutic approach for the impairment of fracture healing.

【 授权许可】

Unknown