Multifunctionality of Nanosized Calcium Apatite Dual-Doped with Li+/Eu3+ Ions Related to Cell Culture Studies and Cytotoxicity Evaluation In Vitro

Biomolecules

Lucyna Mrowczynska¹  Julia Miller²  Agata Piecuch³  Zuzanna Drulis-Kawa⁴  Agata Dorotkiewicz-Jach⁴  Marta Tikhomirov⁵  Blazej Pozniak⁵  Paulina Sobierajska⁶  Rafal J. Wiglusz⁶  Justyna Rewak-Soroczynska⁶ 

[1] Department of Cell Biology, Faculty of Biology, Adam Mickiewicz University in Poznan, Uniwersytetu Poznanskiego 6, 61-614 Poznan, Poland;Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, C. K. Norwida 31, 50-375 Wroclaw, Poland;Department of Mycology and Genetics, Wroclaw University, Przybyszewskiego 63, 51-148 Wroclaw, Poland;Department of Pathogen Biology and Immunology, Wroclaw University, Przybyszewskiego 63, 51-148 Wroclaw, Poland;Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, C. K. Norwida 31, 50-375 Wroclaw, Poland;Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, Poland;

关键词: nanoapatites;    Eu³⁺ and Li⁺ ions;    rare earth ions;    photoluminescence;    cytotoxicity;    in vitro cell culture studies;   

DOI  :  10.3390/biom11091388

来源: DOAJ

【 摘 要 】

Li⁺/Eu³⁺ dual-doped calcium apatite analogues were fabricated using a microwave stimulated hydrothermal technique. XRPD, FT-IR, micro-Raman spectroscopy, TEM and SAED measurements indicated that obtained apatites are single-phased, crystallize with a hexagonal structure, have similar morphology and nanometric size as well as show red luminescence. Lithium effectively modifies the local symmetry of optical active sites and, thus, affects the emission efficiency. Moreover, the hydrodynamic size and surface charge of the nanoparticles have been extensively studied. The protein adsorption (lysozyme, LSZ; bovine serum albumin, BSA) on the nanoparticle surface depended on the type of cationic dopant (Li⁺, Eu³⁺) and anionic group (OH⁻, Cl⁻, F⁻) of the apatite matrix. Interaction with LSZ resulted in a positive zeta potential, and the nanoparticles had the lowest hydrodynamic size in this protein medium. The cytotoxicity assessment was carried out on the human osteosarcoma cell line (U2OS), murine macrophages (J774.E), as well as human red blood cells (RBCs). The studied apatites were not cytotoxic to RBCs and J774.E cells; however, at higher concentrations of nanoparticles, cytotoxicity was observed against the U2OS cell line. No antimicrobial activity was detected against Gram-negative bacteria with one exception for P. aeruginosa treated with Li⁺-doped fluorapatite.

【 授权许可】

Unknown