Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by Mass Spectrometry

Viruses

Peter Gohl¹  Oliver Harzer¹  Joerg Kriegsmann²  Cristina Groh-Mohanu²  Petra Wandernoth²  Katharina Kriegsmann³  Mark Kriegsmann⁴  Martin Daeumer⁵ 

[1] Bioscientia, 576080 Ingelheim, Germany;Center for Histology, Cytology and Molecular Diagnostics Trier, 54296 Trier, Germany;Department of Hematology, Oncology and Rheumatology, University Hospital Heidelberg, 69120 Heidelberg, Germany;Institute of Pathology, University Hospital Heidelberg, 69120 Heidelberg, Germany;Kaiserslautern Medical Laboratory, Institute of Immunology and Genetics, 67655 Kaiserslautern, Germany;

关键词: SARS-CoV2;    COVID19;    mass spectrometry;    severe acute respiratory syndrome coronavirus 2;   

DOI  :  10.3390/v12080849

来源: DOAJ

【 摘 要 】

Background: Amplification of viral ribonucleic acid (RNA) by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) is the gold standard to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Since the initial outbreak, strategies to detect and isolate patients have been important to avoid uncontrolled viral spread. Although testing capacities have been upscaled, there is still a need for reliable high throughput test systems, specifically those that require alternative consumables. Therefore, we tested and compared two different methods for the detection of viral PCR products: rRT-PCR and mass spectrometry (MS). Methods: Viral RNA was isolated and amplified from oro- or nasopharyngeal swabs. A total of 22 samples that tested positive and 22 samples that tested negative for SARS-CoV-2 by rRT-PCR were analyzed by MS. Results of the rRT-PCR and the MS protocol were compared. Results: Results of rRT-PCR and the MS test system were in concordance in all samples. Time-to-results was faster for rRT-PCR. Hands-on-time was comparable in both assays. Conclusions: MS is a fast, reliable and cost-effective alternative for the detection of SARS-CoV-2 from oral and nasopharyngeal swabs.

【 授权许可】

Unknown