Molecular detection of colistin resistance genes (mcr-1, mcr-2 and mcr-3) in nasal/oropharyngeal and anal/cloacal swabs from pigs and poultry

Scientific Reports

Kezong Qi¹  Weina Guo²  Yuan Kang³  Stuart Price³  Patrick Kelly⁴  Patrick Butaye⁴  Zhixing Feng⁵  Yaoyao Wang⁶  Jilei Zhang⁶  Feng Yang⁶  Jinfeng You⁶  Jiawei Wang⁶  Afrah Kamal Yassin⁶  Jing Li⁶  Yi Yang⁶  Min Li⁶  Li Chen⁶  Chengming Wang⁶  Ping Jiang⁷  Jiansen Gong⁸  Chunlian Song⁹ 

[1] Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agricultural University;College of Animal Science, Anhui Science and Technology University;College of Veterinary Medicine, Auburn University;Department of Biosciences, Ross University School of Veterinary Medicine;Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-Products;Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine;Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University;Poultry Institute, Chinese Academy of Agricultural Sciences;Yunnan Agricultural University College of Animal Science & Technology;

DOI  :  10.1038/s41598-018-22084-4

来源: DOAJ

【 摘 要 】

Abstract Antimicrobial resistance against colistin has emerged worldwide and is threatening the efficacy of colistin treatment of multi-resistant Gram-negative bacteria. In this study, PCRs were used to detect mcr genes (mcr-1, mcr-2, mcr-3) in 213 anal and 1,339 nasal swabs from pigs (n = 1,454) in nine provinces of China, and 1,696 cloacal and 1,647 oropharyngeal samples from poultry (n = 1,836) at live-bird markets in 24 provinces. The mcr-1 prevalences in pigs (79.2%) and geese (71.7%) were significantly higher than in chickens (31.8%), ducks (34.6%) and pigeons (13.1%). The mcr-2 prevalence in pigs was 56.3%, significantly higher than in chickens (5.5%), ducks (2.3%), geese (5.5%) and pigeons (0%). The mcr-3 prevalences in pigs (18.7%), ducks (13.8%) and geese (11.9%) were significantly higher than in chickens (5.2%) and pigeons (5.1%). In total, 173 pigs and three chickens were positive for all three mcr genes. The prevalences of the mcr were significantly higher in nasal/oropharyngeal swabs than in the anal /cloacal swabs. Phylogenetic studies identified 33 new mcr-2 variants and 12 new mcr-3 variants. This study demonstrates high prevalences of mcr in pigs and poultry in China, and indicates there is need for more thorough surveillance and control programs to prevent further selection of colistin resistance.

【 授权许可】

Unknown