Identification of Indicators for Preterm Birth Using Retinoid Metabolites

Metabolites

Young-Ah You¹  Young-Ju Kim¹  Sunwha Park¹  AbuZar Ansari¹  Seojeong Park²  Youngjoo Kwon²  Soo-Yeon Hwang²  Ga-In Lee³  Soo-Min Kim³  Jeongae Lee⁴ 

[1] Department of Obstetrics and Gynecology, Ewha Medical Institute and College of Medicine, Ewha Womans University, Seoul 07804, Korea;Department of Pharmacy, Ewha Womans University, Seoul 03760, Korea;Graduate Program in System Health Science and Engineering, Ewha Womans University, Seoul 03760, Korea;MolecularRecognition Research Center, Korea Institute of Science and Technology, Seoul 02792, Korea;

关键词: preterm birth;    metabolomic analysis;    retinoid metabolism;    parturition;    13-cis-retinoic acid;   

DOI  :  10.3390/metabo11070443

来源: DOAJ

【 摘 要 】

Metabolites reflect the biochemical dynamics for the maintenance of pregnancy and parturition. UPLC-Q/TOF-MS and LC-MS/MS metabolomics were performed to identify and validate the plasma metabolomic signatures of preterm birth (PTB). We recruited pregnant women between 16 and 40 weeks 5 days gestational age at Ewha Womans Mokdong Hospital for a nested case-control study. In untargeted UPLC-Q/TOF-MS, score plots of partial least-squares discriminant analysis clearly separated the PTB group from the term birth (TB, n = 10; PTB, n = 11). Fifteen metabolites were significantly different between the two groups, as indicated by a variable importance in projection >1 and p < 0.05. Metabolic pathways involving retinol, linoleic acid, D-arginine, and D-ornithine were associated with PTB. Verification by LC-MS/MS focused on retinol metabolism (TB, n = 39; PTB, n = 20). Retinol levels were significantly reduced in PTB compared to TB, while retinal palmitate, all-trans-retinal, and 13-cis-retinoic acid (¹³cis-RA) significantly increased (p < 0.05). Retinol-binding protein levels were also elevated in PTB. Additionally, all-trans-retinal (AUC 0.808, 95% CI: 0.683–0.933) and ¹³cis-RA (AUC 0.826, 95% CI: 0.723–0.930) showed improved predictions for PTB-related retinol metabolites. This study suggests that retinoid metabolism improves the accuracy of PTB predictions and plays an important role in maintaining pregnancy and inducing early parturition.

【 授权许可】

Unknown