Differential genome-wide DNA methylation patterns in childhood obesity

BMC Research Notes

Moshe Szyf¹  Suzanne King²  Lei Cao-Lei²  David P. Laplante³  Guillaume Elgbeili³ 

[1] Department of Pharmacology and Therapeutics and Sackler Program for Epigenetics and Developmental Psychobiology, McGill University;Department of Psychiatry, McGill University and Douglas Hospital Research Centre;Douglas Hospital Research Centre;

关键词: Prenatal maternal stress;    Genome-wide DNA methylation;    Obesity;    Project Ice Storm;    Immune function;   

DOI  :  10.1186/s13104-019-4189-0

来源: DOAJ

【 摘 要 】

Abstract Objective Exposure to stress during pregnancy may program susceptibility to the development of obesity in offspring. Our goal was to determine whether prenatal maternal stress (PNMS) due to a natural disaster was associated with child obesity, and to compare the DNA methylation profiles in obese versus non-obese children at age 13½ years. Women and their children were involved in the longitudinal natural disaster study—Project Ice Strom, which served as a human model to study PNMS. Blood was collected from 31 children (including five obese children). Infinium HumanMethylation450 BeadChip Array was performed for genome-wide DNA methylation analyses. Results Results demonstrated a well-defined obesity-associated genome-wide DNA methylation pattern. There were 277 CpGs, corresponding to 143 genes, were differentially-methylated. IPA analyses revealed 51 canonical pathways, and enrichment of pathways was involved in immune function. Although no significant association was found between PNMS and child obesity, the preliminary data in the study revealed obesity-associated methylation patterns on a genome-wide level in children.

【 授权许可】

Unknown