Production of biogas: relationship between methanogenic and sulfate-reducing microorganisms

Open Life Sciences

Vítěz Tomáš¹  Kushkevych Ivan²  Bartoš Milan³  Vítězová Monika³ 

[1] Department of Agricultural, Food and Environmental Engineering, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic;Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice753/5, 62500 Brno, Czech Republic;Section of Microbiology and Molecular Biotechnology, Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic;

关键词: biogas;    methane production;    methanogenic microorganisms;    sulfate-reducing bacteria;   

DOI  :  10.1515/biol-2017-0009

来源: DOAJ

【 摘 要 】

The production of high-quality methane depends on many factors, including temperature, pH, substrate, composition and relationship of the microorganisms. The qualitative and quantitative composition of methanogenic and sulfate-reducing microorganisms and their relationship in the experimental bioreactors has never been studied. The aim of this research was to characterize, for the first time, the diversity of the methanogenic microorganisms and sulfate-reducing bacteria, and study their relationship and biogas production in experimental bioreactors. Amplification of 16S rRNA gene fragments was carried out. Purified amplicons were paired-end sequenced on an Illumina Mi-Seq platform. The dominant morphotypes of these microorganisms in the bioreactor were homologous (99%) by the sequences of 16S rRNA gene to the Methanosarcina, Thermogymnomonas, Methanoculleus genera and Archaeon deposited in GenBank. Three dominant genera of sulfate-reducing bacteria, Desulfomicrobium, Desulfobulbus and Desulfovibrio, were detected in the bioreactor. The phylogenetic trees showing their genetic relationship were constructed. The diversity and number of the genera, production of methane, hydrogen sulfide and hydrogen in the bioreactor was investigated. This research is important for understanding the relationship between methanogenic microbial populations and other bacterial physiological groups, their substrate competition and, in turn, can be helpful for controlling methanogenesis in bioreactors.

【 授权许可】

Unknown