Impact of a human gut microbe on Vibrio cholerae host colonization through biofilm enhancement

eLife

Firas S Midani¹  Denise Chac²  Meti D Debela³  Jason B Harris³  Chelsea N Dunmire⁴  Kelsey Barrasso⁴  Abigail Rivera Seda⁴  Jing Yan⁵  Wai-Leung Ng⁶  Anjali Steenhaut⁶  Catherine Geigel⁷  Firdausi Qadri⁷  Regina C Larocque⁷  Ana A Weil⁸ 

[1] Department of Pediatrics, Harvard Medical School, Boston, United States;Program of Molecular Microbiology, Graduate School of Biomedical Sciences, Tufts University School of Medicine, Boston, United States;Department of Medicine, University of Washington, Seattle, United States;Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, United States;Department of Molecular Virology and Microbiology at Baylor College of Medicine, Houston, United States;Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, United States;Division of Infectious Diseases, Massachusetts General Hospital, Boston, United States;International Center for Diarrheal Disease Research, Bangladesh, Dhaka, Bangladesh;

关键词: Vibrio cholerae;    Paracoccus aminovorans;    biofilm;    microbiome;    infectious disease;    pathogenesis;   

DOI  :  10.7554/eLife.73010

来源: DOAJ

【 摘 要 】

Recent studies indicate that the human intestinal microbiota could impact the outcome of infection by Vibrio cholerae, the etiological agent of the diarrheal disease cholera. A commensal bacterium, Paracoccus aminovorans, was previously identified in high abundance in stool collected from individuals infected with V. cholerae when compared to stool from uninfected persons. However, if and how P. aminovorans interacts with V. cholerae has not been experimentally determined; moreover, whether any association between this bacterium alters the behaviors of V. cholerae to affect the disease outcome is unclear. Here, we show that P. aminovorans and V. cholerae together form dual-species biofilm structure at the air–liquid interface, with previously uncharacterized novel features. Importantly, the presence of P. aminovorans within the murine small intestine enhances V. cholerae colonization in the same niche that is dependent on the Vibrio exopolysaccharide and other major components of mature V. cholerae biofilm. These studies illustrate that multispecies biofilm formation is a plausible mechanism used by a gut microbe to increase the virulence of the pathogen, and this interaction may alter outcomes in enteric infections.

【 授权许可】

Unknown