Delivery of rivaroxaban and chitosan rapamycin microparticle with dual antithrombosis and antiproliferation functions inhibits venous neointimal hyperplasia

Drug Delivery

Hualong Bai¹  Hao He²  Liwei Zhang³  Haoliang Wu³  Cong Zhang³  Yuanfeng Liu³  Peng Sun³  Chunyang Lou³  Jingan Li⁴ 

[1] Henan Key Laboratory of Advanced Magnesium Alloy &Department of Vascular Surgery, The Second Xiangya Hospital of Central South University, Changsha, China;Department of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Henan, China;;School of Material Science and Engineering &

关键词: Neointimal hyperplasia;    rivaroxaban;    rapamycin;    chitosan microparticle;    polyglycolic acid;   

DOI  :  10.1080/10717544.2022.2092240

来源: DOAJ

【 摘 要 】

Neointimal hyperplasia is a complex process after vascular interventions, acute platelet deposition and smooth muscle cell proliferation both contributed to this process. There are still no perfect solutions to solve this problem. Rivaroxaban is a novel anticoagulant that has been widely used in clinic, it has a good pharmacological effects both in vivo and in vitro. Chitosan microparticle rapamycin (MP-rapa) was fabricated, interspaces of polyglycolic acid (PGA) scaffold were used as a reservoir of MP-rapa, and the scaffold was coated with hyaluronic acid rivaroxaban (MP-rapa-riva). Scanning electronic microscopy (SEM) photographs were taken and water contact angles were measured, rat inferior vena cava (IVC) patch venoplasty model was used; patches were harvested at day 14 and examined by immunohistochemistry and immunofluorescence. SEM photographs showed the microparticles rapamycin were inside the interspace of the scaffold, hyaluronic acid rivaroxaban was also successfully coated onto the surface of the scaffold. There was a thinner neointima, fewer proliferating cell nuclear antigen (PCNA) positive cells, fewer macrophages in the MP-rapa and MP-rapa-riva grafts compared to the control PGA graft. The result showed that this scaffold with dual anticoagulation and antiproliferation functions can effectively inhibit venous neointimal hyperplasia, although this is an animal experiment, it showed promising potential clinical application in the future.

【 授权许可】

Unknown