Clover Species Specific Influence on Microbial Abundance and Associated Enzyme Activities in Rhizosphere and Non-Rhizosphere Soils

Agronomy

Jakub Elbl¹  Igor Hunady¹  Adnan Mustafa²  Antonin Kintl³  Martin Brtnicky³  Jiri Holatko³  Tereza Hammerschmiedt³  Jiri Skladanka⁴  Tomas Vyhnanek⁵  Jiri Kucerik⁶ 

[1]Agricultural Research, Ltd., Zahradni 400/1, 664 41 Troubsko, Czech Republic

[2]Biology Center CAS, SoWa RI, Na Sadkach 7, 370 05 Ceske Budejovice, Czech Republic

[3]Department of Agrochemistry, Soil Science, Microbiology and Plant Nutrition, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic

[4]Department of Animal Nutrition and Forage Production, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic

[5]Department of Plant Biology, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic

[6]Institute of Chemistry and Technology of Environmental Protection, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic

关键词: rhizosoil;    bulk soil;    soil microbiota;    soil enzymes;    plant biomass;    plant nutrients;   

DOI  :  10.3390/agronomy11112214

来源: DOAJ

【 摘 要 】

Legume cultivation, especially the clover species, has shown promoting effects on soil biological properties. However, the ways in which various clover species contribute to beneficial plant-rhizosphere soil interactions have remained neglected in the past. Therefore, we performed a field experiment to assess and compare the species-specific influence of five different clover species on plant traits, microbial soil health indicators, namely soil enzymes, microbial biomass and abundance and their potential nutrient cycling abilities under rhizosphere and non-rhizosphere soils. For this, soil samples from bulk soil and rhizosphere of each clover species were collected and analyzed for soil enzymes including β-glucosidase, arylsulfatase, phosphatase, N-acetyl-β-D-glucosaminidase, and urease and microbial communities’ abundance. Results revealed that the soil biological properties were more affected in the rhizosoil than in the bulk soil, although the individual legume crop variants differed in the rate and extent of the differential impact on either rhizosoil or bulk soil. The most significantly affected species-specific properties were ammonium oxidizing bacteria and phosphorus-solubilizing microbiota in the rhizosoil of white clover and alsike clover variants, whereas the least impact was exerted by sweet clover. The biological properties of rhizosoil showed a significant effect on the plant qualitative and quantitative properties. We further detected antagonism among N and P + K transfer from the rhizosoil to plants, which influenced above ground and root biomass. Overall, these results suggest that the positive effects of clover species cultivation on rhizosphere soil properties are species specific.

【 授权许可】

Unknown