Development of Clostridium saccharoperbutylacetonicum as a Whole Cell Biocatalyst for Production of Chirally Pure (R)-1,3-Butanediol

Frontiers in Bioengineering and Biotechnology

Dana Heldt¹  Zak Bean¹  Edward Green¹  Alexander Grosse-Honebrink²  Ying Zhang²  Ruth H. M. Cornock²  Klaus Winzer²  Gareth T. Little²  Nigel P. Minton² 

[1] CHAIN Biotechnology Ltd., MediCity, Nottingham, United Kingdom;Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), Biodiscovery Institute, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom;

关键词: Clostridium saccharoperbutylacetonicum;    biotechnology;    phaB;    Allele Coupled Exchange;    (R)-1;    3-butanediol;   

DOI  :  10.3389/fbioe.2021.659895

来源: DOAJ

【 摘 要 】

Chirally pure (R)-1,3-butanediol ((R)-1,3-BDO) is a valuable intermediate for the production of fragrances, pheromones, insecticides and antibiotics. Biotechnological production results in superior enantiomeric excess over chemical production and is therefore the preferred production route. In this study (R)-1,3-BDO was produced in the industrially important whole cell biocatalyst Clostridium saccharoperbutylacetonicum through expression of the enantio-specific phaB gene from Cupriavidus necator. The heterologous pathway was optimised in three ways: at the transcriptional level choosing strongly expressed promoters and comparing plasmid borne with chromosomal gene expression, at the translational level by optimising the codon usage of the gene to fit the inherent codon adaptation index of C. saccharoperbutylacetonicum, and at the enzyme level by introducing point mutations which led to increased enzymatic activity. The resulting whole cell catalyst produced up to 20 mM (1.8 g/l) (R)-1,3-BDO in non-optimised batch fermentation which is a promising starting position for economical production of this chiral chemical.

【 授权许可】

Unknown