Opposite Roles of Tumor Cell Proliferation and Immune Cell Infiltration in Postoperative Liver Metastasis of PDAC

Frontiers in Cell and Developmental Biology

Yi Miao²  Zipeng Lu³  Kai Zhang³  Xumin Huang³  Shangnan Dai³  Lingdi Yin³  Hao Gao³  Guangfu Wang³  Yong Gao³ 

[1] Pancreas Center, First Affiliated Hospital of Nanjing Medical University, Nanjing, China;Pancreas Center, The Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, China;Pancreas Institute, Nanjing Medical University, Nanjing, China;

关键词: pancreatic cancer;    liver metastasis;    weighted gene co-expression network analyses;    immune cell infiltration;    cell proliferation;   

DOI  :  10.3389/fcell.2021.714718

来源: DOAJ

【 摘 要 】

BackgroundRecurrence of liver metastasis after pancreatectomy is often a predictor of poor prognosis. Comprehensive genomic analysis may contribute to a better understanding of the molecular mechanisms of postoperative liver metastasis and provide new therapeutic targets.MethodsA total of 67 patients from The Cancer Genome Atlas (TCGA) were included in this study. We analyzed differentially expressed genes (DEGs) by R package “DESeq2.” Weighted gene co-expression network analysis (WGCNA) was applied to investigate the key modules and hub genes. Immunohistochemistry was used to analyze tumor cell proliferation index and CD4+ T cells infiltration.ResultsFunctional analysis of DEGs between the liver metastatic and recurrence-free groups was mainly concentrated in the immune response. The liver metastasis group had lower immune and stroma scores and a higher TP53 mutation rate. WGCNA showed that the genes in key modules related to disease-free survival (DFS) and overall survival (OS) were mainly enriched in the cell proliferation process and tumor immune response. Immunohistochemical analysis showed that the pancreatic cancer cells of patients with early postoperative liver metastasis had higher proliferative activity, while the infiltration of CD4+ T cells in tumor specimens was less.ConclusionOur study suggested that increased immune cell infiltration (especially CD4+ T cells) and tumor cell proliferation may play an opposite role in liver metastasis recurrence after pancreatic cancer.

【 授权许可】

Unknown