LC-MS Identification and Quantification of Phenolic Compounds in Solid Residues from the Essential Oil Industry

Antioxidants

Costas G. Biliaderis¹  Maria Irakli²  Adriana Skendi²  Paschalina Chatzopoulou²  Elisavet Bouloumpasi² 

[1] Department of Food Science and Technology, Aristotle University of Thessaloniki, P.O. Box 235, 54124 Thessaloniki, Greece;Hellenic Agricultural Organization, Demeter, Plant Breeding and Genetic Resources Institute, P.O. Box 60458, Thermi, 57001 Thessaloniki, Greece;

关键词: phenolic acids;    flavonoids;    solid residues;    essential oil industry;    Lamiaceae;    LC-MS;   

DOI  :  10.3390/antiox10122016

来源: DOAJ

【 摘 要 】

Plant solid residues obtained from the essential oil industry represent a rich source of phenolic compounds with bioactive properties to be used in the food and pharmaceutical industries. A selective and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed for the simultaneous determination of phenolic compounds in solid residues of the Lamiaceae family plants. A total of 48 compounds can be separated within 35 min by using the Poroshell-120 EC-C₁₈ column, and a gradient mobile phase of 0.1% formic acid and acetonitrile with flow rate of 0.5 mL/min; salicylic acid was used as internal standard. The calibration curves showed good linearity in the tested concentration range for each analyte (R² > 0.9921), while recoveries ranged from 70.1% to 115.0% with an intra-day and inter-day precision of less than 6.63% and 15.00%, respectively. Based on the retention behavior, as well as absorption and mass spectra, 17 phenolic acids, 19 flavonoids and 2 phenolic diterpenes were identified and quantified in the solid residues obtained by distillation of six aromatic plants: oregano, rosemary, sage, satureja, lemon balm, and spearmint. The method constitutes an accurate analytical and quality control tool for the simultaneous quantitation of phenolics present in solid waste residues from the essential oil industry.

【 授权许可】

Unknown