CaSBP11 Participates in the Defense Response of Pepper to Phytophthora capsici through Regulating the Expression of Defense-Related Genes

International Journal of Molecular Sciences

Zhen-Hui Gong¹  Xiao-Hui Feng¹  Huai-Xia Zhang¹  Jing-Hao Jin¹  Abid Khan²  Xiao-Hua Du³  Wei-Li Guo³ 

[1] College of Horticulture, Northwest A&F University, Yangling, Xianyang 712100, China;Department of Horticulture, The University of Haripur, Haripur 22620, Pakistan;School of Horticulture Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China;

关键词: pepper;    CaSBP11;    Phytophthora capsici;    defense-related genes;    Nicotiana benthamiana;   

DOI  :  10.3390/ijms21239065

来源: DOAJ

【 摘 要 】

Squamosa promoter binding protein (SBP)-box genes are plant-specific transcription factors involved in plant growth and development, morphogenesis and biotic and abiotic stress responses. However, these genes have been understudied in pepper, especially with respect to defense responses to Phytophthora capsici infection. CaSBP11 is a SBP-box family gene in pepper that was identified in our previous research. Silencing CaSBP11 enhanced the defense response of pepper plants to Phytophthora capsici. Without treatment, the expression of defense-related genes (CaBPR1,CaPO1,CaSAR8.2 and CaDEF1) increased in CaSBP11-silenced plants. However, the expression levels of these genes were inhibited under transient CaSBP11 expression. CaSBP11 overexpression in transgenic Nicotiana benthamiana decreased defense responses, while in Arabidopsis, it induced or inhibited the expression of genes in the salicylic acid and jasmonic acid signaling pathways. CaSBP11 overexpression in sid2-2 mutants induced AtNPR1, AtNPR3, AtNPR4, AtPAD4, AtEDS1, AtEDS5, AtMPK4 and AtNDR1 expression, while AtSARD1 and AtTGA6 expression was inhibited. CaSBP11 overexpression in coi1-21 and coi1-22 mutants, respectively, inhibited AtPDF1.2 expression and induced AtPR1 expression. These results indicate CaSBP11 has a negative regulatory effect on defense responses to Phytophthora capsici. Moreover, it may participate in the defense response of pepper to Phytophthora capsici by regulating defense-related genes and the salicylic and jasmonic acid-mediated disease resistance signaling pathways.

【 授权许可】

Unknown