Microbiome of Odontogenic Abscesses

Microorganisms

Philipp Streckbein¹  Jan-Falco Wilbrand¹  Hans-Peter Howaldt¹  Daniel Schmermund¹  Sameh Attia¹  Michael Knitschke¹  Sebastian Böttger¹  Eugen Domann²  Jörn Pons-Kühnemann³  Torsten Hain⁴  Silke Zechel-Gran⁴  Markus Weigel⁴ 

[1] Department of Oral and Maxillofacial Surgery, Justus-Liebig-University Giessen, University Hospital Giessen and Marburg, Giessen, D-35392 Giessen, Germany;German Center for Infection Research (DZIF), Justus-Liebig-University Giessen, Partner Site Giessen-Marburg-Langen, D-35392 Giessen, Germany;Institute of Medical Informatics, Justus-Liebig-University Giessen, D-35392 Giessen, Germany;Institute of Medical Microbiology, Justus-Liebig-University Giessen, D-35392 Giessen, Germany;

关键词: oral microbiome;    odontogenic abscess;    16S rRNA gene analysis;    polymicrobial infection;    anaerobic infection;   

DOI  :  10.3390/microorganisms9061307

来源: DOAJ

【 摘 要 】

Severe odontogenic abscesses are regularly caused by bacteria of the physiological oral microbiome. However, the culture of these bacteria is often prone to errors and sometimes does not result in any bacterial growth. Furthermore, various authors found completely different bacterial spectra in odontogenic abscesses. Experimental 16S rRNA gene next-generation sequencing analysis was used to identify the microbiome of the saliva and the pus in patients with a severe odontogenic infection. The microbiome of the saliva and the pus was determined for 50 patients with a severe odontogenic abscess. Perimandibular and submandibular abscesses were the most commonly observed diseases at 15 (30%) patients each. Polymicrobial infections were observed in 48 (96%) cases, while the picture of a mono-infection only occurred twice (4%). On average, 31.44 (±12.09) bacterial genera were detected in the pus and 41.32 (±9.00) in the saliva. In most cases, a predominantly anaerobic bacterial spectrum was found in the pus, while saliva showed a similar oral microbiome to healthy individuals. In the majority of cases, odontogenic infections are polymicrobial. Our results indicate that these are mainly caused by anaerobic bacterial strains and that aerobic and facultative anaerobe bacteria seem to play a more minor role than previously described by other authors. The 16S rRNA gene analysis detects significantly more bacteria than conventional methods and molecular methods should therefore become a part of routine diagnostics in medical microbiology.

【 授权许可】

Unknown