Partial Substitution of Meat with Insect (Alphitobius diaperinus) in a Carnivore Diet Changes the Gut Microbiome and Metabolome of Healthy Rats

Foods

Sofie Kaas Lanng¹  Hanne Christine Bertram¹  Dennis Sandris Nielsen²  Yichang Zhang²  Witold Kot³  Kristine Rothaus Christensen⁴  Axel Kornerup Hansen⁴ 

[1] Department of Food Science, Aarhus University, Agro Food Park 48, 8200 Aarhus, Denmark;Department of Food Science, University of Copenhagen, Rolighedsvej 30, 1958 Frederiksberg, Denmark;Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg, Denmark;Department of Veterinary and Animal Sciences, University of Copenhagen, Grønnegårdsvej 15, 1958 Frederiksberg, Denmark;

关键词: NMR-based metabolomics;    microbiota;    insect protein;    protein digestion;    alternative proteins;   

DOI  :  10.3390/foods10081814

来源: DOAJ

【 摘 要 】

Insects are suggested as a sustainable protein source of high nutritional quality, but the effects of insect ingestion on processes in the gastrointestinal tract and gut microbiota (GM) remain to be established. We examined the effects of partial substitution of meat with insect protein (Alphitobius diaperinus) in a four-week dietary intervention in a healthy rat model (n = 30). GM composition was characterized using’ 16S rRNA gene amplicon profiling while the metabolomes of stomach, small intestine, and colon content, feces and blood were investigated by ¹H-NMR spectroscopy. Metabolomics analyses revealed a larger escape of protein residues into the colon and a different microbial metabolization pattern of aromatic amino acids when partly substituting pork with insect. Both for rats fed a pork diet and rats fed a diet with partial replacement of pork with insect, the GM was dominated by Lactobacillus, Clostridium cluster XI and Akkermansia. However, Bray-Curtis dissimilarity metrics were different when insects were included in the diet. Introduction of insects in a common Western omnivore diet alters the gut microbiome diversity with consequences for endogenous metabolism. This finding highlights the importance of assessing gastrointestinal tract effects when evaluating new protein sources as meat replacements.

【 授权许可】

Unknown