Identificaiton of a High-yield Technique for Isolating Endometrial Epithelial Cells from the Mouse Uterus: A Comparison of Mechanical and Sedimentation-adherence Methods

Journal of Animal Reproduction and Biotechnology

Jeongmook Lim¹  Yoonmi Jo¹  Jieohn Sohn¹  Jiyeon Ahn¹  Seungtae Lee¹  Hyunjin Song¹  Hyejin Park¹ 

[1] Fertility Medical Center, Seoul Women's Hospital;

关键词: mouse;    sedimentation-adherence method;    isolation;    endometrial epithelial cells;    uterus;   

DOI  :  10.12750/JET.2016.31.1.73

来源: DOAJ

【 摘 要 】

An in vitro assay following culture of endometrial epithelial cells is essential for understanding epithelial cell function in reproduction. Several diverse techniques have been developed for isolating endometrial epithelial cells, although an optimal technique has not been identified. In this study, we describe a sedimentation-adherence (S-A) isolation technique with a high-yield cell-separating ability to isolate endometrial epithelial cells from 8-week-old female C57BL/6 mice. We analyzed total cell number, viability, morphology, and expression of cytokeratin 18 as an endometrial epithelial cell-specific marker in cells isolated using a mechanical method compared to the S-A technique. There were no significant differences in the total number, viability, or morphology of the putative endometrial epithelial cells with either method. In contrast, significantly more endometrial epithelial cells harvested using the S-A method were positively stained for cytokeratin 18 than those isolated using the mechanical method. These results confirm that the S-A method is more efficient for retrieving endometrial epithelial cells than a mechanical method.

【 授权许可】

Unknown