Single-Cell Elasticity Measurement with an Optically Actuated Microrobot

Micromachines

Judit Molnár¹  Kinga Molnár¹  Tamás Fekete¹  Gaszton Vizsnyiczai¹  Pál Ormos¹  István Grexa¹  Lóránd Kelemen¹ 

[1] Biological Research Centre, Temesvári krt. 62, 6726 Szeged, Hungary;

关键词: cell elasticity;    endothelial cells;    optical micromanipulation;    holographic optical tweezers;    two-photon polymerization;    image processing;   

DOI  :  10.3390/mi11090882

来源: DOAJ

【 摘 要 】

A cell elasticity measurement method is introduced that uses polymer microtools actuated by holographic optical tweezers. The microtools were prepared with two-photon polymerization. Their shape enables the approach of the cells in any lateral direction. In the presented case, endothelial cells grown on vertical polymer walls were probed by the tools in a lateral direction. The use of specially shaped microtools prevents the target cells from photodamage that may arise during optical trapping. The position of the tools was recorded simply with video microscopy and analyzed with image processing methods. We critically compare the resulting Young’s modulus values to those in the literature obtained by other methods. The application of optical tweezers extends the force range available for cell indentations measurements down to the fN regime. Our approach demonstrates a feasible alternative to the usual vertical indentation experiments.

【 授权许可】

Unknown