Detection of Y Chromosome Microdeletions and Hormonal Profile Analysis of Infertile Men undergoing Assisted Reproductive Technologies

International Journal of Fertility and Sterility

Maryam Davari¹  Najmeh Moein Vaziri¹  Afsoon Zarei¹  Bahia Namavar Jahromi²  Somayeh Montazeri³  Shahryar Zeighami³  Mohammad Ebrahim Parsanezhad³  Ardeshir Bahmanimehr⁴ 

[1] 4Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;Depatment of Urology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;Thalassemia and Hemophilia Genetic, PND Research Center, Dastgheib Hospital, Shiraz University of Medical Science, Shiraz, Iran;

关键词: assisted reproductive technologies;    non;    obstructive azoospermia;    y chromosome deletion;   

DOI  :  10.22074/ijfs.2018.5244

来源: DOAJ

【 摘 要 】

BackgroundY chromosome deletions (YCDs) in azoospermia factor (AZF) region are associated with ab-normal spermatogenesis and may lead to azoospermia or severe oligozoospermia. Assisted reproductive tech-nologies (ART) by intracytoplasmic sperm injection (ICSI) and testicular sperm extraction (TESE) are com-monly required for infertility management of patients carrying YCDs. The aim of this study was to estimatethe frequency of YCDs, to find the most frequent variant in infertile men candidate for ART and to compareYCD distribution with a control fertile group. The semen parameters, hormonal profiles and ART outcomesof the infertile group were studied. Materials and MethodsThis case-control study consisted of 97 oligozoospermic or non-obstructive azoospermic(NOA) infertile men, who had undergone ART, as the case group and 100 fertile men as the control group. DNAsamples were extracted from blood samples taken from all 197 participants and YCDs were identified by multiplexpolymerase chain reaction (PCR) of eight known sequence-tagged sites. The chi-square test was used to comparethe mean values of hormone and sperm parameters between the two groups. P < 0.05 was considered statisticallysignificant.ResultsNo YCD was detected in the control group. However, 20 out of 97 (20.6%) infertile men had a YCD. AZFc,AZFbc and AZFabc deletions were detected in 15 (75%), four (20%) and one (5%) YCD-positive patients. No fer-tilization or clinical pregnancy was seen following ICSI in this sub-group with YCD. The mean level of FSH wassignificantly higher in the group with YCD (28.45 ± 22.2 vs. 4.8 ± 3.17 and 10.83 ± 7.23 in YCD-negative patientswith and without clinical pregnancy respectively).ConclusionYCD is frequent among NOA men and YCD screening before ART and patient counseling is thusstrongly recommended.

【 授权许可】

Unknown