3D-printed SAXS chamber for controlled in situ dialysis and optical characterization

Journal of Synchrotron Radiation

Joachim O. Rädler¹  Julian Philipp¹  Achim Theo Brinkop¹  Martin Barkey¹  Martina Ober¹  Bert Nickel¹  David Simml¹  Miriam von Westphalen¹  Tamara Ehm²  Roy Beck² 

[1] Faculty of Physics, Ludwig-Maximilians-Universität, Geschwister-Scholl-Platz 1, Munich, Germany;School of Physics and Astronomy, Center for Physics and Chemistry of Living Systems, and Center for Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, Israel;

关键词: cyclic olefin copolymer;    3d printing;    in situ dialysis;    in-house measurements;    small-angle x-ray scattering;   

DOI  :  10.1107/S1600577522005136

来源: DOAJ

【 摘 要 】

3D printing changes the scope of how samples can be mounted for small-angle X-ray scattering (SAXS). In this paper a 3D-printed X-ray chamber, which allows for in situ exchange of buffer and in situ optical transmission spectroscopy, is presented. The chamber is made of cyclic olefin copolymers (COC), including COC X-ray windows providing ultra-low SAXS background. The design integrates a membrane insert for in situ dialysis of the 100 µl sample volume against a reservoir, which enables measurements of the same sample under multiple conditions using an in-house X-ray setup equipped with a 17.4 keV molybdenum source. The design's capabilities are demonstrated by measuring reversible structural changes in lipid and polymer systems as a function of salt concentration and pH. In the same chambers optical light transmission spectroscopy was carried out measuring the optical turbidity of the mesophases and local pH values using pH-responsive dyes. Microfluidic exchange and optical spectroscopy combined with in situ X-ray scattering enables vast applications for the study of responsive materials.

【 授权许可】

Unknown