Detection of SARS-associated Coronavirus in Throat Wash and Saliva in Early Diagnosis

Emerging Infectious Diseases

关键词: severe acute respiratory syndrome;    SARS;    coronavirus;    CoV;    Taiwan;    perspective;   

DOI  :  10.3201/eid1007.031113

来源: DOAJ

【 摘 要 】

The severe acute respiratory syndrome–associated coronavirus (SARS-CoV) is thought to be transmitted primarily through dispersal of droplets, but little is known about the load of SARS-CoV in oral droplets. We examined oral specimens, including throat wash and saliva, and found large amounts of SARS-CoV RNA in both throat wash (9.58 x 102 to 5.93 x 106 copies/mL) and saliva (7.08 x 103 to 6.38 x 108 copies/mL) from all specimens of 17 consecutive probable SARS case-patients, supporting the possibility of transmission through oral droplets. Immunofluorescence study showed replication of SARS-CoV in the cells derived from throat wash, demonstrating the possibility of developing a convenient antigen detection assay. This finding, with the high detection rate a median of 4 days after disease onset and before the development of lung lesions in four cases, suggests that throat wash and saliva should be included in sample collection guidelines for SARS diagnosis.

【 授权许可】

Unknown