How to extract and analyze pollen from internal organs and exoskeletons of fossil insects?

STAR Protocols

Sonja Wedmann¹  Silvia Ulrich²  Friðgeir Grímsson³  Reinhard Zetter³  Thomas Hörnschemeyer⁴  Michael S. Engel⁵ 

[1]Anthropologie, Georg-August-Universität Göttingen, 37073 Göttingen, Germany

[2]Corresponding author

[3]Department of Botany and Biodiversity Research, University of Vienna, 1030 Vienna, Austria

[4]Department of Paleontology, University of Vienna, 1090 Vienna, Austria

[5]

[6]Johann-Friedrich-Blumenbach-Institut für Zoologie &

关键词: Microscopy;    Plant sciences;    Earth sciences;    Evolutionary biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: This protocol explains how to extract pollen from fossil insects with subsequent descriptions of pollen treatment. We also describe how to document morphological and ultrastructural features with light-microscopy and electron microscopy. It enables a taxonomic assignment of pollen that can be used to interpret flower-insect interactions, foraging and feeding behavior of insects, and the paleoenvironment. The protocol is limited by the state of the fossil, the presence/absence of pollen on fossil specimens, and the availability of extant pollen for comparison.For complete details on the use and execution of this protocol, please refer to Wappler et al. (2015), Ulrich and Grímsson (2020), and Wedmann et al. (2021).

【 授权许可】

Unknown