Open Life Sciences | |
Expression of Zeb1 in the differentiation of mouse embryonic stem cell | |
Wei Wei1  Guo Xin2  Chen Ting2  Yu Zhendong2  Pan Peng2  Cui Guanghui2  Zhang Yanmin2  | |
[1] Department of Blood Vessel Surgical Treatment Area, Changchun Provincial People’s Hospital, 1183 Industrial and Agricultural Road, Changchun, 130021, China;Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, 1120 Lianhua Road, Shenzhen, Guangdong 518036, China; | |
关键词: zeb1; lin28a; embryonic stem cells; mouse embryonal carcinoma cells; | |
DOI : 10.1515/biol-2022-0042 | |
来源: DOAJ |
【 摘 要 】
Embryonic stem cells (ESCs) differentiation is a process of replication and refinement, and the directional lineage differentiation of ESCs involves the epithelial-mesenchymal transition (EMT)- mesenchymal-epithelial transition (MET) process. A previous study revealed that Zinc finger E-box-binding homeobox 1 (Zeb1) plays a vital role in EMT, which could repress E-cadherin promoter and induce an EMT in cells. To verify the expression of Zeb1 and its correlation with Lin28a in mouse ESCs differentiation, we performed qRT-PCR and western blots to detect the expression of Lin28a mRNA and protein after Zeb1 knockdown. The expression of Zeb1 decreased over time of mouse ESCs differentiation but significantly increased in mouse embryonal carcinoma cells. After knockdown of Zeb1, Lin28a and Vimentin expression were decreased, while E-cadherin expression increased both in mouse ESCs, EBs, GC1, and P19 cells. We found that Zeb1 promoted the invasive ability of mouse embryonal carcinoma cells. These results revealed that expression of Zeb1 decreased during the differentiation of ESCs, and Lin28a and EMT processes can be regulated by Zeb1, which need to be verified in the future studies.
【 授权许可】
Unknown