Establishment and Application of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Detection of Shewanella Genus

Frontiers in Microbiology

Hongyan Cai¹  Yue Xiao¹  Keyi Yu¹  Zhenzhou Huang¹  Hang Dai¹  Duochun Wang¹  Ruiting Lan³  Ying Li⁴  Shiyao Wu⁵  Lirong Lin⁵  Qingbo Fu⁵ 

[1] Center for Human Pathogenic Culture Collection, China CDC, Beijing, China;National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), State Key Laboratory for Infectious Disease Prevention and Control, Beijing, China;School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia;Workstation for Microbial Infectious Disease, Shunyi District Center for Disease Control and Prevention, Beijing, China;Zybio Inc., Chongqing, China;

关键词: MALDI-TOF mass spectrometry;    detection;    Shewanella;    multilocus sequence analysis;    establishment and application;   

DOI  :  10.3389/fmicb.2021.625821

来源: DOAJ

【 摘 要 】

Shewanella species are widely distributed in the aquatic environment and aquatic organisms. They are opportunistic human pathogens with increasing clinical infections reported in recent years. However, there is a lack of a rapid and accurate method to identify Shewanella species. We evaluated here matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid identification of Shewanella. A peptide mass reference spectra (PMRS) database was constructed for the type strains of 36 Shewanella species. The main spectrum projection (MSP) cluster dendrogram showed that the type strains of Shewanella species can be effectively distinguished according to the different MS fingerprinting. The PMRS database was validated using 125 Shewanella test strains isolated from various sources and periods; 92.8% (n = 116) of the strains were correctly identified at the species level, compared with the results of multilocus sequence analysis (MLSA), which was previously shown to be a method for identifying Shewanella at the species level. The misidentified strains (n = 9) by MALDI-TOF MS involved five species of two groups, i.e., Shewanella algae–Shewanella chilikensis–Shewanella indica and Shewanella seohaensis–Shewanella xiamenensis. We then identified and defined species-specific biomarker peaks of the 36 species using the type strains and validated these selected biomarkers using 125 test strains. Our study demonstrated that MALDI-TOF MS was a reliable and powerful tool for the rapid identification of Shewanella strains at the species level.

【 授权许可】

Unknown