The nuclear lamina is a hub for the nuclear function of Jacob

Molecular Brain

Anna Karpova¹  Sebastian Samer¹  Michael R. Kreutz¹  Rajeev Raman¹  Gregor Laube¹ 

[1] RG Neuroplasticity, Leibniz Institute for Neurobiology;

关键词: Jacob/Nsmf;    Lamin B1;    CRM1;    Nuclear export;    NMDAR;    Synapse-to-nucleus;   

DOI  :  10.1186/s13041-020-00722-1

来源: DOAJ

【 摘 要 】

Abstract Jacob is a synapto-nuclear messenger protein that couples NMDAR activity to CREB-dependent gene expression. In this study, we investigated the nuclear distribution of Jacob and report a prominent targeting to the nuclear envelope that requires NMDAR activity and nuclear import. Immunogold electron microscopy and proximity ligation assay combined with STED imaging revealed preferential association of Jacob with the inner nuclear membrane where it directly binds to LaminB1, an intermediate filament and core component of the inner nuclear membrane (INM). The association with the INM is transient; it involves a functional nuclear export signal in Jacob and a canonical CRM1-RanGTP-dependent export mechanism that defines the residing time of the protein at the INM. Taken together, the data suggest a stepwise redistribution of Jacob within the nucleus following nuclear import and prior to nuclear export.

【 授权许可】

Unknown