Genetic Susceptibility to Neurodegeneration in Amazon: Apolipoprotein E Genotyping in Vulnerable Populations Exposed to Mercury

Frontiers in Genetics

Jacqueline I. Alvarez-Leite¹  Rosa C. R. Martín-Doimeadios²  María Jiménez-Moreno²  Sergio Fernández-Trujillo²  Liz C. Silva-Pereira³  Reinaldo B. Oriá⁴  José R. Souza-Monteiro⁵  Maria E. Crespo-Lopez⁵  Gabriela P. F. Arrifano⁵  Ândrea Ribeiro-dos-Santos⁶  Marcos T. Amador⁶  Sidney Santos⁶  Marcus Augusto-Oliveira⁷  Barbarella M. Macchi⁸  José L. M. do Nascimento⁹ 

[1] Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;Department of Analytical Chemistry and Food Technology, Faculty of Environmental Sciences and Biochemistry, University of Castilla-La Mancha, Toledo, Spain;Instituto Federal de Educação, Itaituba, Brazil;Laboratório da Biologia da Cicatrização, Ontogenia e Nutrição de Tecidos, Departamento de Morfologia e Instituto de Biomedicina, Escola de Medicina, Universidade Federal do Ceará, Fortaleza, Brazil;Laboratório de Farmacologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Brazil;Laboratório de Genética Humana e Médica, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Brazil;Laboratório de Investigações em Neurodegeneração e Infecção (Hospital Universitário João de Barros Barreto), Universidade Federal do Pará, Belém, Brazil;Laboratório de Neuroquímica Molecular e Celular, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Brazil;Neuroscience Research Group, CEUMA University, São Luís, Brazil;

关键词: ApoE;    Tapajós;    Tucuruí;    human;    methylmercury;    ancestry;   

DOI  :  10.3389/fgene.2018.00285

来源: DOAJ

【 摘 要 】

Human exposure to mercury is a serious problem of public health in Amazon. As in other vulnerable populations throughout the world, Amazonian riverine populations are chronically exposed to this metal and some symptoms of mercury intoxication were already detected in these populations. However, studies on the genetic susceptibility to mercury toxicity in the Amazon are scarce, and they tested a limited number of individuals. In this context, apolipoprotein E gene (APOE) is a key element with a well-established association among their alleles and the neurodegenerative consequences of mercury intoxication. However, no studies have addressed APOE genotyping in Amazonian exposed populations. Additionally, epidemiological studies with APOE genotyping in Amazon have been restricted to indigenous populations. Therefore, this work analyzed for the first time the genotypic and allelic profiles of APOE in Amazonian riverine populations chronically exposed to mercury. Eight hundred and twenty three individuals were enrolled in our study donating blood (794) and/or hair (757). APOE genotyping was analyzed by real-time PCR. Total mercury and mercury species were quantified by ICP-MS and GC-pyro-AFS, respectively. Genomic ancestry markers were evaluated by multiplex-PCR reaction, separated by capillary electrophoresis on the ABI 3130 Genetic Analyzer instrument and analyzed on GeneMapper ID v3.2. The

【 授权许可】

Unknown