Chemical Characterization and Hypoglycaemic Activities In Vitro of Two Polysaccharides from Inonotus obliquus by Submerged Culture

Molecules

Shisheng Tong¹  Jiao Xue²  Ping Liu²  Zhaorun Wang³ 

[1] Bio-Pharmaceutical College, Beijing City University, Beijing 100094, China;College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;School of Life Sciences, Inner Mongolia University, Inner Mongolia 010021, China;

关键词: Inonotus obliquus;    submerged culture;    polysaccharides;    diabetes;    HepG2 cells;   

DOI  :  10.3390/molecules23123261

来源: DOAJ

【 摘 要 】

Polysaccharides from the fungus Inonotus obliquus have been found to be biologically active. In this study, we carried out a preliminary characterisation and assessment of the hypoglycaemic activities of the polysaccharides (IOEP) from Inonotus obliquus obtained by liquid fermentation. Two polysaccharides, IOEP1 and IOEP2, were isolated from IOEP. IOEP1, with a molecular weight of 20 KDa, was mainly composed of galatose and mannose, while IOEP2, with a molecular weight of 200 KDa, was mainly composed of arabinose. Fourier-transform infrared analysis showed that both IOEP1 and IOEP2 were pyran-type polysaccharides. ¹H-NMR spectra showed that the glycosidic bonds of IOEP1 and IOEP2 were both α-type and β-type. In addition, IOEP1 and IOEP2 strongly increased the glucose consumption of HepG2 cells and insulin-resistant HepG2 cells in vitro. These findings provide a theoretical basis that IOEP1 and IOEP2 might be suitable as anti-diabetes agents in functional foods and natural drugs.

【 授权许可】

Unknown