Eicosapentaenoic Acid Extraction from Nannochloropsis gaditana Using Carbon Dioxide at Supercritical Conditions

Marine Drugs

Sanjeet Mehariya¹  Despina Karatza¹  Angela Iovine¹  Dino Musmarra¹  Antonio Molino²  Vincenzo Larocca³  Giuseppe Di Sanzo³  Maria Martino³  Anna Spagnoletta³  Tiziana Marino⁴ 

[1] Department of Engineering, University of Campania “L.Vanvitelli”, Real Casa dell’Annunziata, Via Roma 29, 81031 Aversa, Italy;ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Department of Sustainability-CR Portici, P. Enrico Fermi, 1, 80055 Portici, Italy;ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Department of Sustainability-CR Trisaia, SS Jonica 106, km 419+500, 7026 Rotondella, Italy;Institute on Membrane Technology, National Research Council (ITM-CNR) Via Pietro Bucci, Cubo 17C, 870 36 Rende, Italy;

关键词: microalgae;    Nannochloropsis gaditana;    lipids;    eicosapentaenoic acid (EPA);    Supercritical-CO₂ fluid extraction;    pharmaceutical;    nutraceutical;   

DOI  :  10.3390/md17020132

来源: DOAJ

【 摘 要 】

This research shows that carbon dioxide supercritical fluid (CO₂-SF) is an emerging technology for the extraction of high interest compounds for applications in the manufacturing of pharmaceuticals, nutraceuticals, and cosmetics from microalgae. The purpose of this study is to recover fatty acids (FAs) and, more precisely, eicosapentaenoic acid (EPA) from Nannochloropsis gaditana biomass by CO₂-SF extraction. In the paper, the effect of mechanical pre-treatment was evaluated with the aim of increasing FAs recovery. Extraction was performed at a pressure range of 250⁻550 bars and a CO₂ flow rate of 7.24 and 14.48 g/min, while temperature was fixed at 50 or 65 °C. The effect of these parameters on the extraction yield was assessed at each extraction cycle, 20 min each, for a total extraction time of 100 min. Furthermore, the effect of biomass loading on EPA recovery was evaluated. The highest EPA extraction yield, i.e., 11.50 mg/g, corresponding to 27.4% EPA recovery, was obtained at 65 °C and 250 bars with a CO₂ flow rate of 7.24 g/min and 1.0 g biomass loading. The increased CO₂ flow rate from 7.24 to 14.48 g/min enhanced the cumulative EPA recovery at 250 bars. The purity of EPA could be improved by biomass loading of 2.01 g, even if recovery was reduced.

【 授权许可】

Unknown