Intranasal HD-Ad vaccine protects the upper and lower respiratory tracts of hACE2 mice against SARS-CoV-2

Cell & Bioscience

Natasha Christie-Holmes¹  Betty Poon¹  Karen Mossman²  James M. Rini³  Jun Liu³  Ming Li³  Scott D. Gray-Owen³  Juntao Mai³  Zhichang Zhou³  Jacqueline Watt³  Zhijie Li³  Sang Kyun Ahn³  Rob Kozak⁴  Samira Mubareka⁴  Rongqi Duan⁵  Ranmal Avinash Bandara⁵  Ziyan Chen⁵  Huibi Cao⁵  Jim Hu⁵  Arinjay Banerjee⁶ 

[1] Combined Containment Level 3 Unit, Faculty of Medicine, University of Toronto;Department of Medicine Institute for Infectious Disease Research, McMaster Immunology Research Center, McMaster University;Department of Molecular Genetics, Faculty of Medicine, University of Toronto;Sunnybrook Heath Sciences Centre;Translational Medicine Program, Hospital for Sick Children Research Institute;Vaccine and Infectious Disease Organization, University of Saskatchewan;

关键词: COVID-19;    HD-Ad;    Nasal delivery;    SARS-CoV2;    Vaccine;   

DOI  :  10.1186/s13578-021-00723-0

来源: DOAJ

【 摘 要 】

Abstract Background The ongoing COVID-19 pandemic has resulted in 185 million recorded cases and over 4 million deaths worldwide. Several COVID-19 vaccines have been approved for emergency use in humans and are being used in many countries. However, all the approved vaccines are administered by intramuscular injection and this may not prevent upper airway infection or viral transmission. Results Here, we describe a novel, intranasally delivered COVID-19 vaccine based on a helper-dependent adenoviral (HD-Ad) vector. The vaccine (HD-Ad_RBD) produces a soluble secreted form of the receptor binding domain (RBD) of the SARS-CoV-2 spike protein and we show it induced robust mucosal and systemic immunity. Moreover, intranasal immunization of K18-hACE2 mice with HD-Ad_RBD using a prime-boost regimen, resulted in complete protection of the upper respiratory tract against SARS-CoV-2 infection. Conclusion Our approaches provide a powerful platform for constructing highly effective vaccines targeting SARS-CoV-2 and its emerging variants.

【 授权许可】

Unknown