Biliary Microbial Structure of Gallstone Patients With a History of Endoscopic Sphincterotomy Surgery

Frontiers in Cellular and Infection Microbiology

Ming Ni¹  Fuqiang Ye²  Huijie Lv³  Hongzhang Shen³  Dongchao Xu³  Jianfeng Yang³  Hangbin Jin³  Xiaofeng Zhang³  Qifeng Lou³  Juanjuan Zhu⁴  Liangliang Fang⁵ 

[1] Beijing Institute of Radiation Medicine, Beijing, China;Department of Disease Control and Prevention, Center for Disease Control and Prevention of Eastern Theater Command, Nanjing, China;Department of Gastroenterology, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, China;School of Life Science and Technology, China Pharmaceutical University, Nanjing, China;The First School of Clinical Medicine, Nanjing Medical University, Nanjing, China;

关键词: choledocholithiasis;    bile;    biliary microbiota;    endoscopic sphincterotomy;    amplicon sequencing;   

DOI  :  10.3389/fcimb.2020.594778

来源: DOAJ

【 摘 要 】

The biliary microbiota is related to the pathogenesis of human bile duct stones. However, the extent to which a history of invasive endoscopic sphincterotomy (EST) affects the biliary bacterial community remains largely unknown. We collected bile samples from the common bile duct of 100 choledocholithiasis patients. We performed 16S rRNA sequencing to investigate and compare the biliary microbial community. The patients without antibiotic treatment (AT) were grouped into three clusters based on their biliary microbial compositions. The patients with a history of EST were significantly enriched in one cluster mainly consisting of gastrointestinal bacteria compared with the other two clusters consisting of oral and environmental bacteria. The β-diversities of patients with and without EST were also significantly different, whereas the α-diversities were comparable. The only significantly enriched bacterial genus associated with a history of EST was Pyramidobacter, while eight other genera were significantly decreased. For patients with AT, seven of these genera maintained their association with EST, including Pyramidobacter. However, after AT, the difference in β-diversities was diminished. EST induced a marked shift in the biliary microbial composition. A cluster of biliary bacteria was associated with a history of EST, and Pyramidobacter was specific to EST.

【 授权许可】

Unknown