Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach

Molecules

Rivelino Armando Vera-Sánchez¹  Jesús Alfredo Araujo-León¹  Julio Enrique Oney-Montalvo¹  Tania Isolina Coral-Martínez¹  Zulema Cantillo-Ciau²  Rolffy Ortiz-Andrade³ 

[1] Chromatography Laboratory, Chemistry Faculty, Autonomous University of Yucatan, Mérida 97069, Mexico;Medicinal Chemistry Laboratory, Chemistry Faculty, Autonomous University of Yucatan, Mérida 97069, Mexico;Pharmacology Laboratory, Chemistry Faculty, Autonomous University of Yucatan, Mérida 97069, Mexico;

关键词: ESI;    flavonoid;    optimization;    pharmacokinetic;    validation;    QqQ;   

DOI  :  10.3390/molecules25184241

来源: DOAJ

【 摘 要 】

The purpose of this study was to develop, optimize, and fully validate a high-sensitivity methodology using UHPLC-MS/MS to simultaneously quantify hesperidin and naringenin in microsamples (100 µL) of murine plasma after intragastric administration of single pure flavonoids and a mixture. The optimization process allowed for high sensitivity with detection limits of approximately picogram order using an electrospray ionization (ESI) source in negative mode and an experiment based on multiple reaction monitoring (MRM). The validation parameters showed excellent linearity and detection limits, with a precision of less than 8% and a recovery of over 90%. This methodology was applied to compare the pharmacokinetic parameters for the administration of hesperidin and naringenin in individual form or in the form of a mixture. The results showed an absence of significant effects (p > 0.05) for Tmax and Cmax; however, the AUC presented significant differences (p < 0.05) for both flavonoids when administered as a mixture, showing an improved absorption ratio for both flavonoids.

【 授权许可】

Unknown