Analytical Technique Optimization on the Detection of β-cyclocitral in Microcystis Species

Molecules

Keisuke Kanei¹  Suzue Arii¹  Beata Bober¹  Ryuji Yamashita²  Ken-ichi Harada²  Kiyomi Tsuji³ 

[1] Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku. Nagoya 468-8503, Japan;Graduate School of Environmental and Human Science, Meijo University, 150 Yagotoyama, Tempaku, Nagoya 468-8503, Japan;Kanagawa Prefectural Institute of Public Health, 1-3-1 Shimomachiya, Chigasaki, Kanagawa 253-0087, Japan;

关键词: analysis;    microcystis;    spme;    volatile organic compounds;   

DOI  :  10.3390/molecules25040832

来源: DOAJ

【 摘 要 】

β-Cyclocitral, specifically produced by Microcystis, is one of the volatile organic compounds (VOCs) derived from cyanobacteria and has a lytic activity. It is postulated that β-cyclocitral is a key compound for regulating the occurrence of cyanobacteria and related microorganisms in an aquatic environment. β-Cyclocitral is sensitively detected when a high density of the cells is achieved from late summer to autumn. Moreover, it is expected to be involved in changes in the species composition of cyanobacteria in a lake. Although several analysis methods for β-cyclocitral have already been reported, β-cyclocitral could be detected using only solid phase micro-extraction (SPME), whereas it could not be found at all using the solvent extraction method in a previous study. In this study, we investigated why β-cyclocitral was detected using only SPME GC/MS. Particularly, three operations in SPME, i.e., extraction temperature, sample stirring rate, and the effect of salt, were examined for the production of β-cyclocitral. Among these, heating (60 °C) was critical for the β-cyclocitral formation. Furthermore, acidification with a 1-h storage was more effective than heating when comparing the obtained amounts. The present results indicated that β-cyclocitral did not exist as the intact form in cells, because it was formed by heating or acidification of the resulting intermediates during the analysis by SPME. The obtained results would be helpful to understand the formation and role of β-cyclocitral in an aquatic environment.

【 授权许可】

Unknown