Analysis of Light-Induced Changes in the Photochemical Reflectance Index (PRI) in Leaves of Pea, Wheat, and Pumpkin Using Pulses of Green-Yellow Measuring Light

Remote Sensing

Ekaterina Sukhova¹  Vladimir Sukhov¹ 

[1] Department of Biophysics, N.I. Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603950, Russia;

关键词: green-yellow light pulses;    NPQ;    nonphotochemical quenching;    photochemical reflectance index;    photosynthesis;    plant;    PRI;    remote sensing;   

DOI  :  10.3390/rs11070810

来源: DOAJ

【 摘 要 】

The photochemical reflectance index (PRI) is a widely used spectral index which can show stress-induced changes in photosynthesis (e.g., increase of the nonphotochemical quenching of chlorophyll fluorescence (NPQ)). The artificial illumination of plants improves the efficiency of estimation of photosynthetic processes on the basis of PRI measurements. However, the simultaneous activity of different light sources with different locations can disturb the measurement of PRI. Using pulses of a green-yellow measuring light can potentially solve this problem. The aim of the present work was to investigate the possibility of using green-yellow light pulses for the investigation of light-induced changes in PRI in higher plants (pea, wheat, and pumpkin) and for the analysis of connection between PRI and the energy-dependent component of NPQ (NPQ_F). First, we showed that using green-yellow light pulses eliminated shifts of reflected light, which were connected with the application of a red actinic light. Second, analysis of light dependences of NPQ_F, the absolute value of PRI, and changes in PRI (the difference between the PRI under the actinic light and the initial value of PRI without this light, ΔPRI) showed that the dynamics of the increase of NPQ_F and the decrease of PRI and ΔPRI were similar. Changes in NPQ_F and ΔPRI were found to be significant. In contrast, changes in the absolute value of PRI were not significant in most of the variants of the experiments. Third, scatter plots between NPQ_F and ΔPRI showed similar linear correlations for investigated species; moreover, a total set of experimental points (for pea, wheat, and pumpkin) were also described by the same linear regression. Thus, our results show that (i) pulses of green-yellow measuring light can be used for measurements of PRI, and (ii) ΔPRI is a more effective indicator for the estimation of NPQ than the absolute value of PRI.

【 授权许可】

Unknown