Phosphorylated Radix Cyathulae officinalis Polysaccharides Act as Adjuvant via Promoting Dendritic Cell Maturation

Molecules

Sean P. McDonough¹  Yung-Fu Chang¹  Shiping Yang²  Xuelian Zhao²  Haibo Feng²  Yong Lu²  Yun Gan²  Xiao Yi²  Jing Fan³ 

[1] College of Veterinary Medicine, Cornell University, Ithaca, NY 14850, USA;Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, China;Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610051, China;

关键词: Radix Cyathulae polysaccharide;    phosphorylation modification;    adjuvant;    dendritic cell;   

DOI  :  10.3390/molecules22010106

来源: DOAJ

【 摘 要 】

The aim of this study was to investigate whether phosphorylated Radix Cyathulae officinalis Kuan polysaccharides (pRCPS) used as adjuvant with foot-and-mouth disease vaccine (FMDV) can stimulate specific humoral and cellular immune responses in ICR mice. The results demonstrated that pRCPS significantly up-regulated FMDV-specific IgG, IgG1, IgG2b and IgG2a antibody levels and splenocyte proliferation. pRCPS also promoted the killing activities of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). In addition, pRCPS enhanced the expression levels of IL-2, IL-4, and IFN-γ in CD4+ T cells and the level of IFN-γ in CD8+ T cells. Importantly, pRCPS enhanced the expression of MHCII, CD40+, CD86+, and CD80+ in dendritic cells (DCs). This study indicated that phosphorylation modification could increase immune-enhancing activities of RCPS, and pRCPS could promote humoral and cellular immune responses through facilitating DC maturation.

【 授权许可】

Unknown