Functional and Activity Analysis of Cattle UCP3 Promoter with MRFs-Related Factors

International Journal of Molecular Sciences

Zhongwei Liu¹  Xiang Chen²  Wei Chen²  Dan Xia²  Wen Zhang²  Houqiang Xu² 

[1] College of Life Science, Guizhou University, Guiyang 550025, China;Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Guizhou University, Guiyang 550025, China;

关键词: uncoupling protein 3 (UCP3);    promoter;    transcriptional activity;    MRFs family;    myocyte-specific enhancer factor 2A (MEF2A);   

DOI  :  10.3390/ijms17050682

来源: DOAJ

【 摘 要 】

Uncoupling protein 3 (UCP3) is mainly expressed in muscle. It plays an important role in muscle, but less research on the regulation of cattle UCP3 has been performed. In order to elucidate whether cattle UCP3 can be regulated by muscle-related factors, deletion of cattle UCP3 promoter was amplified and cloned into pGL3-basic, pGL3-promoter and PEGFP-N3 vector, respectively, then transfected into C2C12 myoblasts cells and UCP3 promoter activity was measured using the dual-Luciferase reporter assay system. The results showed that there is some negative-regulatory element from −620 to −433 bp, and there is some positive-regulatory element between −433 and −385 bp. The fragment (1.08 kb) of UCP3 promoter was cotransfected with muscle-related transcription factor myogenic regulatory factors (MRFs) and myocyte-specific enhancer factor 2A (MEF2A). We found that UCP3 promoter could be upregulated by Myf5, Myf6 and MyoD and downregulated by MyoG and MEF2A.

【 授权许可】

Unknown